Table 1

Strains and constructs used in the project

Strain or antisense expt hostEpidemic type (spa gene) or plasmidReference, source, or NCBI accession no. of genome sequence
MRSA strains
    S. aureus N315Japan-New York ST5 (t002)NC_002745
    S. aureus Mu50Japan-New York ST5 (t002)NC_002758
    S. aureus COLST250NC_002951
    S. aureus LT2981-04ST225 (t003) PFGE Rhine-HesseCP001844
    S. aureus Sanger 252ST30BX571856
    S. aureus USA100ST5 (t002)NARSA: NRS382
    S. aureus LT3391-02ST5 (t002) PFGE Rhine-HesseGerman Reference Center for Staphylococci, Wernigerode, Germany
    S. aureus LT1450-94Northern German, Iberian ST247German Reference Center for Staphylococci, Wernigerode, Germany
    S. aureus LT825-96Berlin ST45German Reference Center for Staphylococci, Wernigerode, Germany
    S. aureus LT831-96Southern German ST228German Reference Center for Staphylococci, Wernigerode, Germany
    S. aureus LT635-93Vienna ST239German Reference Center for Staphylococci, Wernigerode, Germany
    S. aureus LT1000-93Hannover ST254German Reference Center for Staphylococci, Wernigerode, Germany
    S. aureus LT2321-01Barnim EMRSA15 ST22German Reference Center for Staphylococci, Wernigerode, Germany
    S. aureus LT2040-05USA300 ST8German Reference Center for Staphylococci, Wernigerode, Germany
    111 MRSA strainsClinical MRSA isolatesThis study
    136 MRSA strainsClinical MRSA isolates (blind group)This study
    38 outbreak strainsMainly MRSA14
MSSA strains
    S. aureus NCTC8325 (RN1)Laboratory MSSA, rsbU-negative ST8, t211NC_007795
    S. aureus NCTC8325-4 (RN0450)NCTC8325 after curation of phages, rsbU-negativeNARSA: NRS135
    S. aureus SH1000S. aureus NCTC 8325-4 rsbU repaired24
    S. aureus RN4220Restriction-negative derivative of NCTC8325-4NARSA: NRS144
    S. aureus ReynoldsMSSA ST25NARSA: NRS102
    S. aureus Cowan I (S11)MSSA ST30NCTC: NCTC 8530
    S. aureus NewmanMSSA ST254AP009351
    95 MSSA strainsClinical MSSA isolatesThis study
Antisense expt host
    S. aureus RN4220pEPSA5-AS-SAS078This study
    S. aureus Sanger 252pEPSA5-AS-SAR1012This study
    S. aureus RN4220, S. aureus N315pEPSA5-AS-SA0772This study
    S. aureus RN4220pEPSA5-AS-SA2039This study
    S. aureus RN4220pEPSA5-AS-SA1414This study
    S. aureus RN4220pEPSA5-AS-SA1305This study