Table 3

PCR/ESI-MS results for formalin-fixed paraffin-embedded heart valve tissues from subjects with negative valvular Gram stainsb

Valve typeaBlood culture resultValve culture resultTime from positive blood culture to surgery (days)Age of FFPE block (yr)PCR/ESI-MS resultLevelcQ score
PMSSAMSSA613Staphylococcus aureus (mecA negative)220.99
NMSSANegative5211S. aureus (mecA negative)70.98
NMSSANegative262S. aureus (mecA negative)1040.99
NVGSNegative1212Streptococcus gordonii791
PVGSNegative8411Streptococcus sp.30.97
NPeptostreptococcus sp.Negative604Gemella morbillorumd1780.98
NGranulicatella sp.Negative114G. adiacens1690.99
Candida tropicalis430.98
NEscherichia coliNegative154E. coli2560.99
Staphylococcus epidermidis300.97
PMSSANegative1211Propionibacterium acnese220.98
PCoNSNegative1712P. acnese780.99
PMSSANegative3210No detection
NMSSANegative2411No detection
NS. epidermidisCoNS513No detection
PCoNSNegative2112No detection
NVGSNegative2012No detection
PEnterococcus faecalisNegative2112No detection
NCardiobacterium hominisNegative2212No detection
NE. faecalisNegative614No detection
NE. faecalisNegative324No detection
NPositive Bartonella serology and PCRNegative04No detection
  • a Infection of a native (N) or prosthetic (P) valve.

  • b MSSA, methicillin-susceptible S. aureus; VGS, viridans group Streptococcus sp.; CoNS, coagulase-negative Staphylococcus sp.

  • c The amount of amplified DNA present in the sample, reported as genome equivalents/well.

  • d Synonym, “Peptostreptococcus morbillorum.”

  • e Discordant with previous microbiology.