Table 2

PCR/ESI-MS assay results for formalin-fixed paraffin-embedded heart valve tissues from subjects with positive valvular Gram stainsb

Valve typeaBlood culture resultValve culture resultTime from positive blood culture to surgery (days)Age of FFPE block (yr)PCR/ESI-MS resultLevelcQ score
PMSSAMSSA612Staphylococcus aureuse671
PMSSANegative2511S. aureuse41
PMSSANegative1811S. aureuse3491
NMSSAMSSA811S. aureuse1891
NMSSAMSSA411S. aureuse1260.99
NMSSANegative510S. aureuse1611
NMRSAMRSA410S. aureus611
mecA positive271
NMRSAS. aureus211S. aureus1741
mecA positive31
PNot doneS. aureus (oxacillin susceptibility unknown)NAd12S. aureuse190.99
PMSSANegative144S. aureuse830.99
NMSSANegative284S. aureuse2691
NMSSANegative313S. aureuse810.99
NMSSANegative534S. aureuse70.98
NMRSAMRSA23S. aureus670.99
mecA positive1440.98
PStaphylococcus epidermidis (oxacillin susceptible)Negative2012S. epidermidise1481
NVGSNegative1013Streptococcus sp.60.97
NNegativeVGSNA13Streptococcus oralis841
NNo cultures obtainedVGSNA12Streptococcus mutans460.98
PVGSNegative84Streptococcus sanguinis2330.99
NStreptococcus mitisNegative23S. mitis group450.98
NVGSNegative02S. mitis group50.98
NS. mutansNegative253S. mutans770.98
NStreptococcus bovis groupNegative712S. equinus/bovis complex831
PCardiobacterium hominisNegative30811C. hominis2330.99
NEnterococcus faecalis (vancomycin susceptible)E. faecalis610E. faecalisf1741
NE. faecalis (vancomycin susceptible)Negative511E. faecalisf2311
NE. faecalis (vancomycin susceptible)Negative1111E. faecalisf620.99
NEnterococcus faecium (vancomycin resistant)VGS213E. faecium831
vanA positive391
NE. faecalisE. faecalis132E. faecalis1580.99
NStaphylococcus lugdunensis (methicillin susceptible)Not done53S. lugdunensise770.98
NCoNS (methicillin susceptible)Negative54S. lugdunensise2250.99
NCoNS (methicillin-resistant)CoNS84S. epidermidis1620.99
mecA positive2520.98
PCoNS (methicillin-resistant)Negative14S. epidermidis/Staphylococcus caprae1161
mecA positive1760.98
PCoNS (methicillin-resistant)Negative104S. epidermidis2110.99
mecA positive1810.98
PPropionibacterium sp.Negative664P. acnes2640.99
NHaemophilus parainfluenzaeNegative214H. parainfluenzae4240.99
Candida tropicalis60.98
NAerococcus sp.Negative84Granulicatella adiacens940.97
Aerococcus urinae1030.98
C. tropicalis460.98
NVGSNegative44Streptococcus pneumoniae1640.99
C. tropicalisg160.98
NVGSNegative1611S. pneumoniaeg1230.97
NS. mitisNegative812S. pneumoniaeg140.94
NS. mitisNegative311S. pneumoniaeg1010.97
PGranulicatella elegansNegative04S. epidermidise650.99
C. tropicalisg420.96
NE. faecalisNegative594S. epidermidise490.99
C. tropicalisg370.97
PS. lugdunensisNot done74Tropheryma whippleig5020.99
NMSSANegative1912No detection
PS. epidermidisNegative512No detection
NVGSNegative1312No detection
NVGSNegative11011No detection
NVGSNegative911No detection
NVGSNegative1312No detection
NS. mitis (VGS)Negative2212No detection
NStreptococcus salivarius (VGS)Negative6611No detection
NS. salivarius (VGS)Negative13512No detection
NH. parainfluenzaeH. parainfluenzae812No detection
PStreptomyces sp.Negative311No detection
PMSSANegative134No detection
PCoNSNegative604No detection
NS. epidermidisNegative63No detection
NVGSNegative32No detection
NS. mitisNegative21No detection
NE. faecalisNegative52No detection
NE. faecalisNegative942No detection
  • a Infection of a native (N) or prosthetic (P) valve.

  • b MSSA, methicillin-susceptible S. aureus; MRSA, methicillin-resistant S. aureus; VGS, viridans group Streptococcus sp.

  • c The amount of amplified DNA present in the sample, reported as genome equivalents/well.

  • d NA, not applicable.

  • e The sample was mecA negative.

  • f The sample was vanA/vanB negative.

  • g Discordant with previous microbiology.