Table 2

Primers used for CRISPR-MVLST PCR and sequencing

MarkerOrientationPrimer sequence (5′–3′)Annealing temp (°C)aPrimer used for:
PCRSequencing
CRISPR1-5ForwardTGAAAACAGACGTATTCCGGTAGATT55.5YesYes
CRISPR1-1ReverseCAGCATATTGACAAGGCGCTYesYes
CRISPR1-6ForwardAATTGGGTAGATTTAGGGTGTYes
CRISPR2-3ForwardATTGTTGCGATTATGTTGGT57YesYes
CRISPR2-1ReverseTCCAGCTCCCTTATGATTTTYes
CRISPR2-4ReverseGCAATACCCTGATCCTTAACGCCAYes
fimH-1ForwardAGGTGAACTGTTCATCCAGTGG56.7YesYes
fimH-2ReverseGCGGGCTGAACAAAACACAAYesYes
sseL-1ForwardAAAATCAGGTCTATGCCTGATTTAATATATC60Yes
sseL-5ReverseGGCTCTAAGTACTCACCATTACTYes
sseL-3ForwardACCAGGAAACAGAGCAAAATGAATATATGTYes
sseL-4ForwardTTCTCTCGGTAAACTATCCTATTGGGCYes
  • a The annealing temperature is shown for the PCR primers.