Table 2

Position and frequency of point mutations identified in each gene of interest

GenePositionaMutationAmino acid changeNo. of first samplesb (n = 143 for each gene)Frequency (%)b,cReference no.d
23S rRNA
2071 (2058)A→C10.725
A→G2114.725
A→T21.430
2072 (2059)A→G3826.625
    Total62/14343.4
parC
234C→T*No change27*18.9*
241G→TGly→Cys 81(78)10.735
244G→AAsp→Asn 82(79)10.736
247A→CSer→Arg 83(80)10.734
248G→TSer→Ile 83(80)117.735
259G→AAsp→Asn 87(84)32.135
G→CAsp→His 87(84)10.734
G→TAsp→Tyr 87(84)21.434
260A→GAsp→Gly 87(84)10.735
307G→AeVal→Ile 103(100)e10.7
351T→C*No change1*0.7*
    Total22/14315.4
gyrA
237G→T*No change1*0.7*
270C→T*No change1*0.7*
285G→A*No change4*2.8*
G→CMet→Ile 95(83)10.735, 37
    Total1/1430.7
  • a Position numbers are given according to the M. genitalium G37 genome (GenBank accession no. NC_000908.2). E. coli numbering is shown in parentheses.

  • b Asterisks indicate mutations that were not included in the total due to lack of an amino acid change.

  • c Frequencies are total number of mutations, irrespective of whether a mutation is associated with antibiotic resistance.

  • d Reference for identification of the mutation associated with resistance at the same position in M. genitalium or a closely related organism.

  • e Amino acid position not previously associated with antibiotic resistance.