Table 3

Validation of identification results for arthroconidial yeasts (ascomycetous [Geotrichum-like] or basidiomycetous [Trichosporon-like])

Set and ID resultsaNo. of isolates for:
Routine ID methodITS/LSU sequencingMALDI-TOF MS
Reference CBS set of ascomycetous yeast spp. (n = 40)
    Correct genus and species IDNot performed4040
    Minor error (correct genus with incorrect species ID)00
    Major error (incorrect genus ID)00
    No ID00
Clinical set of ascomycetous yeast spp. (n = 32)
    Correct genus and species ID52828
    Minor error (correct genus with incorrect species ID)2200
    Major error (incorrect genus ID)2 Geotrichum candidum isolates were Magnusiomyces; 3 Geotrichum spp. were Candida spp.00
    No ID04 (new species)4 (new species)
Reference CBS set of basidiomycetous yeast spp. (n = 45)
    Correct genus and species IDNot performed4541
    Minor error (correct genus with incorrect species ID)04 closely related Trichosporon spp., T. ovoides/T. inkin, T. japonicum/T. asteroides, T. dermatis/T. mucoides
    Major error (incorrect genus ID)00
    No ID00
Clinical set of basidiomycetous yeast spp. (n = 102)
    Correct genus and species ID829494
    Minor error (correct genus with incorrect species ID)188 closely related Trichosporon spp., T. ovoides/T. inkin, T. japonicum/T. asteroides, T. asahii/T. faecale8 closely related Trichosporon spp., T. ovoides/T. inkin, T. japonicum/T. asteroides, T. asahii/T. faecale
    Major error (incorrect genus ID)2b00
    No ID000
  • a ID, identification.

  • b The routine identification test results were incorrect, as MALDI-TOF MS and sequencing data are consistent.