TABLE 1

Routine methods for detection of gastrointestinal pathogens and targets represented on two commercial multiplex assays

TargetaMethod(s) used for routine testing and discordant analysisTarget included on multiplex panelb
FilmArrayLuminex
AeromonasCultureIUO
CampylobacterCulture, real-time PCR
Clostridium difficile toxin A/B tcdC geneReal-time PCR
Plesiomonas shigelloidesCulture
SalmonellaCulture, real-time PCR
Yersinia enterocoliticaCulture, real-time PCRRUO
Vibrio spp.CultureRUO
EAECNDc
EPECND
ETECND
STECReal-time PCR
E. coli 0157Culture, real-time PCR
EIECd/ShigellaCulture, real-time PCR
CryptosporidiumAntigen, microscopy
Cyclospora cayetanensisMicroscopy
Entamoeba histolyticaMicroscopyRUO
Giardia lambliaAntigen
Adenovirus 40/41eReal-time PCRRUO
Norovirus GI/GIIeND
Rotavirus AeAntigen
SapoviruseND
AstroviruseND
  • a EAEC, enteroaggregative E. coli; EPEC, enteropathogenic E. coli; ETEC, enterotoxigenic E. coli (lt/st); STEC, Shiga-like toxin-producing E. coli (stx1/stx2); EIEC, enteroinvasive E. coli.

  • b IUO, investigational use only; RUO, research use only.

  • c ND, not done.

  • d The Luminex panel does not specifically target EIEC; however, the Luminex Shigella assay does cross-react with EIEC.

  • e These targets were also tested at the Minnesota Department of Health by individual real-time PCR tests.