TABLE 2

Comparison of two commercial multiplex panels for routine tests using prospective stool samples (n = 230)a

TargetbFilmArraycLuminexFilmArraydLuminexe
TNTPFPFNTNTPFPFNSensitivity (95% CI)Specificity (95% CI)Sensitivity (95% CI)Specificity (95% CI)
Aeromonas spp.84100100 (16.7, 100)100 (94.8, 100)
Campylobacter spp.227300227300100 (38.3, 100)100 (98, 100)100 (38.3, 100)100 (98, 100)
Clostridium difficile toxin A/B19824702002361100 (83.7, 100)96.6 (93.0, 98.5)95.8 (78.1, 99.9)97.2 (93.8, 98.8)
Plesiomonas shigelloides88000ND100 (95, 100)
Salmonella spp.228110229100100 (16.7, 100)99.6 (97.3, 99.9)100 (16.7, 100)100 (98, 100)
Yersinia enterocolitica230000230000ND100 (98, 100)ND100 (98, 100)
Vibrio spp.5000ND100 (51.1, 100)
Vibrio cholerae230000230000ND100 (98, 100)ND100 (98, 100)
EAEC0000NDND
EPEC0000NDND
ETEC227200227200100 (38.3, 100)100 (98, 100)100 (29, 100)100 (98, 100)
STEC229010230000ND99.6 (97.3, 100)ND100 (98, 100)
E. coli 0157230000230000ND100 (98, 100)ND100 (98, 100)
Shigella/EIEC228200227210100 (29, 100)100 (98, 100)100 (29, 100)99.6 (97.3, 99.9)
Cryptosporidium spp.228100229100100 (16.7, 100)100 (98, 100)100 (16.7, 100)100 (98, 100)
Cyclospora cayetanensis15000ND100 (76.1, 100)
Entamoeba histolytica230000230000ND100 (98, 100)ND100 (98, 100)
Giardia lamblia229100228100100 (16.7, 100)100 (98, 100)100 (16.7, 100)100 (98, 100)
Adenovirus 40/41227110229100100 (16.7, 100)99.6 (97.3, 100)100 (16.7, 100)100 (98, 100)
Norovirus GI/GII21611111981220091.7 (62.5, 100)99.5 (97.2, 100)100 (71.8, 100)90.8 (86.2, 94.0)
Rotavirus A229010227020ND99.6 (97.3, 100)ND99.1 (96.7, 100)
Sapovirus01300100 (73.4, 100)ND
Astrovirus0400100ND
  • a The reference standard for a positive result was defined as an organism detected by (i) bacterial culture or microscopy or (ii) ≥2 methods. Empty cells, not present on the panel.

  • b EAEC, enteroaggregative E. coli; EPEC, enteropathogenic E. coli; ETEC, enterotoxigenic E. coli (lt/st); STEC, Shiga-like toxin-producing E. coli (stx1/stx2; E. coli 0157); EIEC, enteroinvasive E. coli.

  • c TN, true negative; TP, true positive; FP, false positive; FN, false negative.

  • d FilmArray yielded additional detections for Aeromonas (n = 3), Vibrio spp. (n = 1), EAEC (n = 4), EPEC (n = 2), Cryptosporidium (n = 1), C. difficile (n = 1), adenovirus (n = 1), norovirus (n = 1), and rotavirus (n = 1) that were not confirmed due to the lack of a confirmatory test or insufficient remaining sample volume. These specimens were not included in the calculation of performance characteristics. CI, confidence interval; ND, not done.

  • e Luminex yielded additional detections for Giardia (n = 1) and rotavirus (n = 1) that were not confirmed due to the lack of a confirmatory method or insufficient remaining sample volume. These specimens were not included in the calculation of performance characteristics.