TABLE 2

Drug resistance mutation profiles and interpretation in RT sequences of discordant plasma-DBS genotyping resultsa

Sample ID (n = 21)bMutation profile in:Drug resistance profile inc:% NTd difference in RTPL VL (log10 copies/ml)e
PlasmaDBSPlasmaDBS
Different DRM profiles and identical interpretations
    Lab D 2-149100IL 103N103NEFVr NVPrEFVr NVPr0.54.7
    Lab E 3-341184V 190A103S 184V 190A3TCr EFVr FTCr NVPr3TCr EFVr FTCr NVPr1.04.4
DRMs in plasma sequences only
    Lab F 2-493103NEFVr NVPr3.05.2
    Lab C 2-423106M 227FLEFVr NVPr3.04.3
    Lab E 2-29298G 106A 184V 227L3TCr FTCr NVPr3.93.2
    Lab F 3-307103N 184V3TCr EFVr FTCr NVPr3.03.8
    Lab F 1-62267DN 90I 181V 184V3TCr ETRr FTCr RPVr3.04.5
    Lab F 1-701103KN 181CY 184MV 190A3TCr EFVr FTCr NVPr RPVr3.74.5
    Lab F 1-252101R 179I 181C 184IV 190AG3TCr EFVr ETRr FTC NVPr RPVr4.03.5
    Lab F 1-60065N 75M 90I 181C 184IMV3TCr d4Tr EFVr FTCr NVPr RPVr2.04.0
DRMs in DBS sequences only
    Lab C 4-471103KNEFVr NVPr1.05.2
Different DRM profiles and interpretations
    Lab B 2-129103N 184V 221HY103N 184V3TCr EFVr FTCr NVPr RPVr3TCr EFVr FTCr NVPr0.74.0
    Lab E 2-055101Q 179A 181C 184V101KQ 179A 181C 184V 221HY3TCr EFVr FTCr NVPr RPVr3TCr EFVr ETRr FTCr NVPr RPVr1.04.1
    Lab B 3-175101HQ 106AV 179I 184V 190A 225HP101Q 106AV 179I 184V 190A 225HP3TCr EFVr ETRr FTCr NVPr3TCr EFVr FTCr NVPr1.05.8
    Lab B 1-320103N 184V 215ST 225H103N 184V 225H3TCr AZTr d4Tr EFV FTCr NVPr3TCr EFVr FTCr NVPr1.04.6
    Lab E 3-120103N 138T 179L 181C 184V 221Y101R 138A3TCr EFVr ETRr FTCr NVPr RPVrETRr4.93.6
    Lab B 3-16198G 181C 184V 215F41LM 98G 181C 184V 215F3TCr AZTr d4Tr EFVr FTCr NVPr RPVr3TCr ABCr AZTr d4Tr EFVr FTCr NVPr RPVr3.04.4
    Lab E 2-558179I 181C 184V 215Y41LM 69ADNT 179I 181C 184V 215Y3TCr AZTr d4Tr EFVr FTCr NVPr RPVr3TCr ABCr AZTr d4Tr DDIr EFVr FTCr NVPr RPVr TDFr1.04.4
    Lab F 2-45175M 77L 101KQ 118I 179I 184V 190A 210W 215Y75M 77L 179I 184V 190A 215Y3TCr ABCr AZTr d4Tr EFVr FTCr NVPr3TCr AZTr d4Tr EFVr FTCr NVPr1.05.0
    Lab A 1-22441L 103N 108I 184V 210LW 215Y41L 103N 108I 184V 215Y3TCr ABCr AZTr d4Tr EFVr FTCr NVPr TDFr3TCr ABCr AZTr d4Tr EFVr FTCr NVPr0.65.6
    Lab C 1-17990IV 179I 181CFGV 184V 215FY 221Y41L 90I 179I 181V 184V 215Y 221Y3TCr AZTr d4Tr EFVr ETRr FTCr NVPr RPVr3TCr ABCr AZTr d4Tr ETRr FTCr RPVr2.04.1
  • a RT, reverse transcriptase; DBS, dried blood spot.

  • b ID, identification; DRMs, drug resistance mutations.

  • c Interpretation algorithm used was the Agence Nationale de Recherches sur le Sida et les Hépatites Virales (ANRS) version 21. 3TC, lamivudine, d4T, stavudine, AZT, zidovudine, NVP, nevirapine, EFV, efavirenz; FTC, emtricitabine; ABC, abacavir; DDI, didanosine; TDF, tenofovir; ETR, etravirine; RPV, rilpivirine.

  • d NT, nucleotide.

  • e PL, plasma.