TABLE 1

Validation of the SAMBA semi-Q cut-off with diluted plasma samples containing HIV-1 subtype Ca

SampleNo. of HIV-1 RNA copies/ml% with positive SAMBA HIV-1 semi-Q result (>1,000 copies/ml)
1151,114100
15,111100
1,511100
7560
1510
150
230,543100
3,054100
1,527100
3050
310
30
3222,238100
22,224100
2,222100
1,111100
22225
220
4121,102100
12,110100
1,21175
60650
1210
120
  • a Four plasma samples were serially diluted to achieve concentrations of viral RNA ranging from 3 (0.48 log10) to 222,238 (5.35 log10) copies/ml according to quantification with Roche TaqMan v2. Four replicates of each dilution were tested with the SAMBA semi-Q.