TABLE 2

Primer sequences, the sizes of the amplified regions, and the annealing temperature for the final seven selected MLST genes

MLST genePrimerPCR product length (bp)MLST region length (bp)Annealing temperature (°C)
ForwardReverse
aarF5′-TTCCATGCAGATATGCACC-3′3′-CCACTCACTAATAGTGTAGC-5′65050052
dfp5′-GATCCGGTACGCTTTATCAG-3′3′-CATAACGGCTGACAATCTCG-5′54745559
galR5′-ATTGGTAACGGTTACCATG-3′3′-GTTGGGCTGAACATATTGGT-5′64850059
glnS5′-GAATCATGTATCCGTGATG-3′3′-GCACAGAAATAACCTTCAC-5′55744256.5
hemA5′-ATGACTCTGCTCGCATTAGG-3′3′-CGGTTGGCAATAATCATATG-5′60249054
speA5′-ATGTCTGATGATAACTTGATT-3′3′-CAGATAAACTTTATGGCCC-5′55045255.5
rfaE5′-ATGAAAGTCACTCTGCCTGA-3′3′-ATCACTGCCTTTAGGATC-5′50942955.5