TABLE 4

Detection of MAP DNA in cultures by IS900 qPCR, comparing the ethanol precipitation and optimized magnetic bead DNA isolation methods

Bactec GI (wk to 999)No. of samplesQuantity of MAP DNAa (pg) detected by:
Ethanol precipitation methodMagnetic bead method
MeanRangeMeanRange
4140.060.005–0.2861,516570–3,415
5200.110.016–0.4511,998779–4,636
6150.040.001–0.1611,390394–2,086
7130.020.003–0.0542,204937–5,328
820.020.015–0.0181,099975–1,222
  • a Determined from the standard curve included in each qPCR experiment.