TABLE 1

Testing results of specimens/isolates associated with the shigellosis outbreak

Sample IDaSample collection date (mo/day/yr)Culture report date (mo/day/yr)FilmArray testing date (mo/day/yr)RIDOH PFGE upload date (mo/day/yr)bDemographicsShigella resultePFGE patterng
Age group (yr)SexcPatient statusdCxFAPCRf
1407/8/20137/11/20137/11/20137/15/20131-5MOP+++J16X01.0408
1467/8/20137/10/20137/11/20137/15/201313-21FOP+++J16X01.0981
1537/8/20137/10/20137/11/20137/18/20131-5MED+++J16X01.0408
1557/9/20137/13/20137/11/2013NA22-64FOP++NA
1567/10/20137/13/20137/11/2013NA1-5FOP++NA
1587/10/20137/13/20137/11/20137/22/20131-5FOP+++J16X01.0408
1607/10/20137/13/20137/11/2013NA1-5MOP++NA
1787/15/20137/18/20137/15/20137/22/20136-12FED+++J16X01.0981
1847/15/20137/19/20137/16/2013NA1-5MED++NA
1857/15/20137/18/20137/16/20137/22/20131-5FOP+++J16X01.0408
  • a ID, identification.

  • b NA, not available.

  • c M, male; F, female.

  • d OP, outpatient; ED, emergency department.

  • e Shigella testing results by stool culture (Cx), FilmArray GI multiplex test (FA), and PCR are shown. The FilmArray assay was unable to differentiate Shigella from EIEC.

  • f Independent PCR targeted the ipaH (invasive plasmid antigen H) gene (specific for Shigella/EIEC), followed by nucleotide BLAST analysis of bidirectioanl sequencing results. PCR testing was performed off-site by BioFire Diagnostics, Inc.

  • g Pulsed field gel electrophoresis (PFGE) types J16X01.0408 and J16X01.0981 were the two dominant types found during the outbreak in Rhode Island but were not present in Rhode Island in the 6 months preceding July 2013. NA, not available.