TABLE 1

Detection limits of the UpE and Orf1A real-time RT-PCR and the MERs-CoV-ICA on camel nasal swabs

DilutionPCR resultCT valueaICA kit result
OriginalPositive27.08Positive
21Positive30.78Positive
22Positive31.15Positive
23Positive31.20Positive
24Positive31.34Positive
25Positive31.42Positive
26Positive32.03Positive
27Positive32.22Negative
28Positive32.83Negative
29NegativeNegative
210NegativeNegative
211NegativeNegative
212NegativeNegative
  • a CT, threshold cycle.