Sample | Biopsy date (mo/yr) | Farm location | Sheep no.^{a} | Treponeme(s) isolated^{b} | PCR result | |||||
---|---|---|---|---|---|---|---|---|---|---|

PCR specific for group^{c}: | Treponema PCR | F. necrophorum | D. nodosus | |||||||

1 | 2 | 3 | ||||||||

1 | 02/09 | Cheshire | 51 | G2S1F | + | + | + | + | + | − |

2 | 02/09 | Cheshire | 52 | G2S2R | + | + | + | + | + | − |

3 | 02/09 | Cheshire | 53 | G2S3R1 | + | + | + | + | + | − |

4 | 02/09 | Cheshire | 54 | G2S4F | + | + | + | + | + | − |

5 | 02/09 | Cheshire | 55 | IF | + | − | + | + | + | − |

6 | 08/09 | Gloucestershire | 11 | G1OV11 | + | + | − | + | − | − |

7 | 08/09 | Gloucestershire | 14 | IF | + | − | + | + | + | − |

8 | 08/09 | Gloucestershire | 17 | IF | − | + | + | + | + | − |

9 | 08/09 | Gloucestershire | 18 | IF | + | − | + | + | + | + |

10 | 08/09 | Gloucestershire | 20 | IF | − | + | + | + | + | + |

11 | 08/09 | Gloucestershire | 21 | IF | + | + | + | + | − | + |

12 | 08/09 | Gloucestershire | 22 | IF | + | − | − | + | − | + |

13 | 01/10 | Cheshire | 28 | IF | − | − | + | + | + | + |

14 | 01/10 | Cheshire | 29 | IF | + | + | − | + | + | + |

15 | 05/13 | Anglesey | 1 | G2SL1 | + | + | + | + | − | + |

16 | 06/13 | Anglesey | 97 | IF | − | + | − | + | − | + |

17 | 06/13 | Anglesey | 73 | IF | − | + | − | + | − | + |

18 | 06/13 | Anglesey | 30 | G2SL5 | + | + | + | + | + | + |

19 | 06/13 | Anglesey | 63 | G12F2 | + | + | + | + | + | + |

20 | 06/13 | Anglesey | 229 | G12F2, G23F1 | + | + | + | + | + | + |

21 | 06/13 | Anglesey | 36 back left | IF | + | + | + | + | + | + |

22 | 06/13 | Anglesey | 36 back right | IF | + | + | + | + | − | − |

23 | 06/13 | Anglesey | 2 | IF | + | + | + | + | + | + |

24 | 06/13 | Denbighshire | 3 | G16F2, G26F1 | + | + | − | + | + | − |

25 | 07/13 | Conwy farm 1 | 218 | IF | − | + | + | + | − | − |

26 | 07/13 | Conwy farm 1 | 10 | G2F2C10, G2ST24 | − | + | − | + | + | + |

27 | 07/13 | Conwyfarm 1 | 49 | G2F3C12, G2F3 | + | + | + | + | + | − |

28 | 07/13 | Conwy farm 1 | 4 | G2F4C4 | + | + | + | + | + | − |

29 | 07/13 | Conwy farm 1 | 53 | IF | + | + | + | + | + | − |

30 | 07/13 | Conwy farm 1 | 12 | G2F6C6 | + | + | + | + | + | − |

31 | 07/13 | Conwy farm 1 | 33 | G1F7C5 | + | + | + | + | + | − |

32 | 07/13 | Conwy farm 1 | 8 | IF | + | + | + | + | + | − |

33 | 07/13 | Conwy farm 1 | 86 | G1F9C27, G2F9 | + | + | + | + | − | − |

34 | 07/13 | Conwy farm 1 | 85 | G2F10C10 | + | + | + | + | + | − |

35 | 07/13 | Conwy farm 1 | 62 | G2F11C11 | + | + | + | + | − | − |

36 | 07/13 | Conwy farm 1 | 96 | IF | + | + | + | + | + | − |

37 | 08/13 | Conwy farm 2 | 5 | G2138C | + | + | + | + | − | + |

38 | 08/13 | Conwy farm 2 | 6 | G2148C | − | + | + | + | + | + |

39 | 08/13 | Conwy farm 2 | 900 | G2158C | − | + | + | + | + | + |

40 | 08/13 | Conwy farm 2 | 930 | IF | + | + | + | + | + | + |

41 | 08/13 | Anglesey | 38 | IF | − | + | − | + | − | + |

42 | 08/13 | Anglesey | 653 | IF | + | − | − | + | + | + |

43 | 08/13 | Anglesey | 58 | IF | + | + | − | + | + | + |

44 | 08/13 | Anglesey | 40 | IF | − | + | − | + | + | + |

45 | 08/13 | Anglesey | 74 | IF | + | + | + | + | + | + |

46 | 08/13 | Anglesey | 60 | G21C11 | − | + | − | + | + | − |

47 | 08/13 | Anglesey | 59 | G22C4 | + | + | + | + | − | − |

48 | 08/13 | Anglesey | 41 | IF | − | + | − | + | − | + |

49 | 08/13 | Anglesey | 39 | IF | + | + | − | + | + | + |

50 | 08/13 | Anglesey | 651 | IF | + | − | + | + | + | + |

51 | 08/13 | Anglesey | 652 | IF | − | + | − | + | + | − |

52 | 08/13 | Anglesey | 33 | IF | − | + | + | + | − | + |

53 | 12/13 | Cheshire | 101* | G21LJ | − | + | − | + | + | + |

54 | 12/13 | Cheshire | 102* | IF | − | + | − | + | − | − |

55 | 12/13 | Cheshire | 103 front left* | G23LJ | + | + | + | + | + | − |

56 | 12/13 | Cheshire | 103 back right* | IF | + | − | + | + | + | − |

57 | 07/14 | Shropshire | 1 | G3ST1 | − | − | + | + | − | + |

58 | 07/14 | Shropshire | 4 | G3S4S | − | + | + | + | + | + |

↵a Sheep number given with additional foot information if animal had multiple feet sampled. Sheep from which healthy foot tissue was also obtained and investigated in this study are indicated by an asterisk. The corresponding results are shown in Table 3.

↵b All isolations are shown for comparison to PCR results. Abbreviation: IF, isolation failed. If isolation was successful, the strain isolated is listed.

↵c Groups 1, 2, and 3 are “T. medium/T. vincentii-like,” “T. phagedenis-like,” and T. pedis spirochetes, respectively, which are routinely found in bovine DD lesions.