TABLE 2

PCR detection of treponemes, D. nodosus, and F. necrophorum in CODD lesion biopsy samples

SampleBiopsy date (mo/yr)Farm locationSheep no.aTreponeme(s) isolatedbPCR result
PCR specific for groupc:Treponema PCRF. necrophorumD. nodosus
123
102/09Cheshire51G2S1F+++++
202/09Cheshire52G2S2R+++++
302/09Cheshire53G2S3R1+++++
402/09Cheshire54G2S4F+++++
502/09Cheshire55IF++++
608/09Gloucestershire11G1OV11+++
708/09Gloucestershire14IF++++
808/09Gloucestershire17IF++++
908/09Gloucestershire18IF+++++
1008/09Gloucestershire20IF+++++
1108/09Gloucestershire21IF+++++
1208/09Gloucestershire22IF+++
1301/10Cheshire28IF++++
1401/10Cheshire29IF+++++
1505/13Anglesey1G2SL1+++++
1606/13Anglesey97IF+++
1706/13Anglesey73IF+++
1806/13Anglesey30G2SL5++++++
1906/13Anglesey63G12F2++++++
2006/13Anglesey229G12F2, G23F1++++++
2106/13Anglesey36 back leftIF++++++
2206/13Anglesey36 back rightIF++++
2306/13Anglesey2IF++++++
2406/13Denbighshire3G16F2, G26F1++++
2507/13Conwy farm 1218IF+++
2607/13Conwy farm 110G2F2C10, G2ST24++++
2707/13Conwyfarm 149G2F3C12, G2F3+++++
2807/13Conwy farm 14G2F4C4+++++
2907/13Conwy farm 153IF+++++
3007/13Conwy farm 112G2F6C6+++++
3107/13Conwy farm 133G1F7C5+++++
3207/13Conwy farm 18IF+++++
3307/13Conwy farm 186G1F9C27, G2F9++++
3407/13Conwy farm 185G2F10C10+++++
3507/13Conwy farm 162G2F11C11++++
3607/13Conwy farm 196IF+++++
3708/13Conwy farm 25G2138C+++++
3808/13Conwy farm 26G2148C+++++
3908/13Conwy farm 2900G2158C+++++
4008/13Conwy farm 2930IF++++++
4108/13Anglesey38IF+++
4208/13Anglesey653IF++++
4308/13Anglesey58IF+++++
4408/13Anglesey40IF++++
4508/13Anglesey74IF++++++
4608/13Anglesey60G21C11+++
4708/13Anglesey59G22C4++++
4808/13Anglesey41IF+++
4908/13Anglesey39IF+++++
5008/13Anglesey651IF+++++
5108/13Anglesey652IF+++
5208/13Anglesey33IF++++
5312/13Cheshire101*G21LJ++++
5412/13Cheshire102*IF++
5512/13Cheshire103 front left*G23LJ+++++
5612/13Cheshire103 back right*IF++++
5707/14Shropshire1G3ST1+++
5807/14Shropshire4G3S4S+++++
  • a Sheep number given with additional foot information if animal had multiple feet sampled. Sheep from which healthy foot tissue was also obtained and investigated in this study are indicated by an asterisk. The corresponding results are shown in Table 3.

  • b All isolations are shown for comparison to PCR results. Abbreviation: IF, isolation failed. If isolation was successful, the strain isolated is listed.

  • c Groups 1, 2, and 3 are “T. medium/T. vincentii-like,” “T. phagedenis-like,” and T. pedis spirochetes, respectively, which are routinely found in bovine DD lesions.