TABLE 3

PCR detection of treponemes, D. nodosus and F. necrophorum in healthy sheep foot tissue biopsies

SampleBiopsy dateFarm locationaSheep no.bPCR result
Specific PCR for groupc:Treponema PCRF. necrophorumD. nodosus
123
109/13Meirionydd1 front left++
209/13Meirionydd1 front right++
309/13Meirionydd1 back left++
409/13Meirionydd1 back right++
509/13Meirionydd2 front left+
609/13Meirionydd2 front right+
709/13Meirionydd2 back left+
809/13Meirionydd2 back right+
909/13Meirionydd3 front left++
1009/13Meirionydd3 front right++
1109/13Meirionydd3 back left+
1209/13Meirionydd3 back right++
1309/13Meirionydd4 front left++
1409/13Meirionydd4 front right+
1509/13Meirionydd4 back left+
1609/13Meirionydd4 back right+
1712/13Cheshire101 front left*++
1812/13Cheshire101 back left*++
1912/13Cheshire101 back right*++
2012/13Cheshire102 front left*++
2112/13Cheshire102 front right*++
2212/13Cheshire102 back right*+
2312/13Cheshire103 front right*++
2412/13Cheshire103 back left*+
2503/1426 front left+
2603/1426 front right+
2703/1426 back left
2803/1426 back right
2903/1483 front left++
3003/1483 front right+
3103/1431 front left+
3203/1431 back left+
3303/1489 front left
3403/1489 back left
3504/148
3604/145
3704/146
3804/1479 front left
3904/1479 back right
4004/1480 front left
4104/1480 front right
4204/1480 back left
4304/1480 back right+
4404/147 front left
4504/147 front right++
4604/147 back left++
4704/147 back right++
4804/1487 front left+++
4904/1487 front right++
5004/1487 back left
5104/1487 back right+
5204/14987 front left++
5304/14987 front right+
5404/14987 back left++
5504/14987 back right++
5604/149
  • a Farm location listed for sheep foot samples not obtained from sheep at the abattoir.

  • b Sheep number given with additional foot information if animal had multiple feet sampled. Sheep which also had a CODD lesion present on a different foot were also investigated in this study (indicated by an asterisk) with corresponding results shown in Table 2.

  • c Groups 1, 2, and 3 are “T. medium/T. vincentii-like,” “T. phagedenis-like,” and T. pedis spirochetes, respectively, which are routinely found in bovine DD lesions.