TABLE 3

qPCR of subgingival plaque, saliva, and sputum samples in the CF patientsa

PatientResult of oprL qPCRResult of qPCR for gyrB and ecfXP. aeruginosa qPCR quantification (CFU/ml)
Subgingival plaqueSalivaSputumSubgingival plaqueSalivaSputumSubgingival plaqueSalivaSputum
NC 1+NMNM000
NC 2+NMNM000
NC 3NMNMNM000
NC 4+NMNM000
NC 5+NMNM000
CC 1++++04.2 · 1032.6 · 106
CC 2++++++1.0 · 1034.8 · 1054.5 · 109
CC 3+++±+1.2 · 10101.1 · 107
CC 4++++++1.12 · 1062.1 · 1071.7 · 108
CC 5++++001.2 · 109
  • a NC, patients not colonized by P. aeruginosa; CC, patients chronically colonized by P. aeruginosa; −, negative detection result; +, positive detection result; ±, positive for gyrB and negative for ecfX; NM, determination not made.