TABLE 4

Performance of Aspergillus PCR when testing serum and plasma samples

ParameteraSerumPlasma
Single PCR positive thresholdMultiple (≥2) PCR positive thresholdSingle PCR positive thresholdMultiple (≥2) PCR positive threshold
Sensitivity (no./total no., % [95% CI])13/19, 68.4 (46.0–84.6)8/19, 42.1 (23.1–63.7)18/19, 94.7 (75.4–99.1)13/19, 68.4 (46.0–84.6)
Specificity (no./total no., % [95% CI])32/42, 76.2 (61.5–86.5)40/42, 95.2 (84.2–98.7)35/42, 83.3 (69.4–91.7)42/42, 100 (85.1–100)
Positive LR2.98.85.7>68.4a
Negative LR0.40.60.060.32
DOR7.514.795.0>213.8a
  • a 95% CI, 95% confidence interval; LR, likelihood ratio; DOR, diagnostic odds ratio. Positive LR and DOR values were calculated with a specificity of 99% to overcome ∞ that would be generated with 100% specificity.