TABLE 2

Aspergillus PCR performance using plasma samples spiked with different fungal loadsa

Sample no.Burden (ge/ml)95% sample variancePositivity rate (%)Mean Cq (95% CI)SE
11,000939.6–1,060.410032.4 (30.7–34.0)0.70
210080.8–119.210036.1 (34.1–38.1)0.84
35036.4–53.610036.9 (35.1–38.7)0.75
4104.0–16.010039.00 (37.1–40.9)0.79
550.8–9.210039.9 (37.8–42.0)0.88
610–2.928.340.0bNAc
7, 80 ×2NA0No signalNA
  • a Plasma samples 1 to 8 were spiked with different fungal burdens (genome equivalents [ge]/ml of plasma).

  • b Plasma 6 was positive in one center in a single sample only.

  • c NA, not available.