TABLE 3

Influence of sample volume (0.1 to 0.5 ml) on Aspergillus real-time PCR crossing points when testing plasma samples containing a range of A. fumigatus genomic DNA concentrations (5 to 1,000 ge/ml)a

Plasma vol (ml)Fungal load (ge/ml of plasma)
510501001,000
0.140.6839.7037.4336.4533.21
0.239.9038.9236.6535.6732.42
0.339.1138.1435.8634.8931.64
0.438.3337.3535.0834.1130.86
0.537.5536.5734.3033.3230.07
  • a The prediction of Cq values was calculated using a linear mixed model.