TABLE 4

Agreement between genotypic and phenotypic methods used for grouping carriage isolates (n = 93)

WGS capsular genogroup assignmentrt-PCR capsular genogroup agreement (%)Phenotypic method agreement (%)a
SASG1SASG2Dot blotFACSbLCPA
MnB (n = 17)1002424243535
MnC (n = 4)10000000
MnW (n = 9)10000112222
MnY (n = 14)1007971867979
NG, ctrA negative (n = 39)72727910010092
NG, ctrA positive (n = 10)1060100100100100
  • a Pairwise concordance rates (Clopper-Pearson exact method) for serogroup assignment by FACS, dot blotting, or LCPA versus WGS were ≤20.4% (95% CI, 11.0 to 30.1%) and that for rt-PCR versus WGS was 47.3% (95% CI, 36.9 to 57.9%).

  • b Four samples were not tested by FACS.