TABLE 3

Sensitivity and specificity of TAC assays on clinical specimens compared to individual real-time PCR assaysa

PathogenNo. of samples% TAC sensitivity (95% CI)% TAC specificity (95% CI)Source(s) of the positivesb
IRTP positive and TAC positiveIRTP positive and TAC negativeIRTP negative and TAC positiveIRTP negative and TAC negative
Chikungunya002216NAc99 (97–100)NA
CCHF000218NA100 (98–100)NA
Dengue379820380 (66–91)96 (93–98)Studies 1 and 6
Ebola4900266100 (93–100)100 (99–100)Study 2
Bundibugyo000208NA100 (98–100)NA
Sudan000217NA100 (98–100)NA
Hantavirus000209NA100 (98–100)NA
Hepatitis E329020678 (62–89)100 (98–100)Study 1
Marburg000213NA100 (98–100)NA
Nipah000217NA100 (98–100)NA
ONNV000218NA100 (98–100)NA
Rift Valley fever71021588 (47–100)100 (98–100)Study 1
West Nile000180NA100 (98–100)NA
Yellow fever000219NA100 (98–100)NA
Bartonella spp.100217100 (3–100)100 (98–100)Study 6
Brucella spp.861120557 (29–82)95 (91–97)Studies 1 and 6
Coxiella burnetii406216100 (40–100)97 (94–99)Study 6
Leptospira spp.3011204100 (29–100)95 (91–97)Studies 3 and 4
Rickettsia spp.242821192 (75–99)96 (93–98)Studies 4 and 6
Salmonella enterica71522388 (47–100)98 (95–99)Studies 1, 5, and 6
Salmonella Typhi41222580 (28–99)99 (97–100)Studies 1, 5, and 6
Yersinia pestis000218NA100 (98–100)NA
Leishmania spp.0202150 (0–84)100 (98–100)Study 1
Plasmodium spp.10261214394 (88–98)92 (87–96)Studies 1, 3, 5, and 6
Trypanosoma brucei000179NA100 (98–100)NA
Total2783765526188 (84–92)99 (98–99)
  • a No cutoff was applied, and the two tests were run for 45 cycles. A sample was called positive when any of the duplicate reactions yielded amplification (Cq < 45).

  • b Studies 1 to 6 are listed in Table 2.

  • c NA, not applicable.