TABLE 2

Details of C. coli isolates with atypical mapA/ceuE primer and probe sequences

Atypical target and isolateST (CC)aGene allele/primer and probe combination (RT-PCR result)bmapA/ceuE RT-PCR result
mapAceuE
mapA
    OXC73526973 (ST1150)20/2 (+)136/22 (+)Mixed
    OXC6987825 (ST828)88/1 (+)3/22 (+)Mixed
    OXC63951487 (ST1150)19/22d (−)17/22 (+)C. coli
    OXC76156760 (UAc)111/23e (late +)139/22 (+)Inconclusive
ceuE
    OXC72416698 (UA)96/19 (−)153/25 (late +)Inconclusive
    OXC72436698 (UA)96/19 (−)153/25 (late +)Inconclusive
    OXC76536975 (UA)114/21 (−)183/26 (late +)Inconclusive
  • a CC, clonal complex.

  • b +, target detected; −, target not detected; late +, target detected after cycle 30.

  • c UA, ST not assigned to a clonal complex.

  • d Forward primer C. jejuni specific, probe and reverse primer C. coli specific.

  • e Forward primer and probe C. jejuni specific, reverse primer C. coli specific.