TABLE 1

Distribution of organisms identified by conventional methods and the FilmArray meningitis/encephalitis (FA ME) panel

Organism identificationaConventional detection, no.FA ME panel detection, no.Baseline agreement, no.Resolution result, no.bSensitivity, % (95% CI)cSpecificity, % (95% CI)c
FA+/R+FA+/R−FA−/R+
Bacteria
    H. influenza454100100 (47.8–100)100 (97.4–100)
    S. pneumoniae363210100 (47.8–100)99.3 (96.1–100)
    S. agalactiae15122166.7 (9.4–99.2)98.6 (95.0–99.8)
    Escherichia coli111NAdNANA100 (2.5–100)100 (97.5–100)
    Listeria monocytogenes001NANANANA100 (97.5–100)
    Neisseria meningitides111NANANA100 (2.5–100)100 (97.5–100)
    Bacteria not in the FA ME panele707NANANANANA
Viruses
    EV37373610197.4 (86.2–99.9)100 (69.2–100)
    HSV-112131102192.9 (66.1–99.8)98.0 (89.1–99.9)
    HSV-2292929NANANA100 (88.1–100)100 (82.4–100)
    HHV-613181260194.7 (74.0–99.9)100 (92.6–100)
    VZV323232NANANA100 (89.1–100)100 (79.4–100)
    CMV74400357.1 (18.4–90.1)100 (91.4–100)
    EBV13251159194.1 (71.3–99.9)84.2 (72.1–92.5)
    PV010100100 (2.5–100)100 (92.5–100)
Yeast
    C. neoformans/gattii1498100f64.3 (35.1–87.2)NA
Total1741861611914892.8 (88.2–96.0)92.8 (88.2–96.0)
  • a Organisms detected by the FA ME panel include Escherichia coli K1, Haemophilus influenza, Listeria monocytogenes, Neisseria meningitides, Streptococcus agalactiae, Streptococcus pneumoniae, cytomegalovirus (CMV), enterovirus (EV), Epstein-Barr virus (EBV), herpes simplex virus 1 (HSV-1), herpes simplex virus 2 (HSV-2), human herpesvirus 6 (HHV-6), human parechovirus (PV), varicella-zoster virus (VZV), and Cryptococcus neoformans/Cryptococcus gattii. The FA ME assay does not differentiate cryptococcal species.

  • b FA, FilmArray; R, discrepancy resolution nucleic acid test result; +, positive result; −, negative result.

  • c Calculated sensitivity and specificity takes in to account discrepancy resolution testing. CI, confidence interval.

  • d NA, not applicable or not able to calculate.

  • e Bacteria isolated in culture but not included in the panel were Citrobacter koseri, Klebsiella pneumoniae, and Providencia stuartii as well as possible skin contaminants Staphylococcus epidermidis, Staphylococcus haemolyticus, and Staphylococcus warneri.

  • f Six cryptococcal antigen-positive, FA ME-negative specimens were also negative for yeast by DNA sequence analysis.