Summary of urine cultivation protocols for catheterized urine specimens

ProtocolVol (μl) of urineMedium or mediaConditionsIncubation(s) (h) for microbial identificationPatient sample identifiera
Standard urine culture1BAP, MacConkey agarAerobic, 35°C241–107
Modified urine culture1BAP, MacConkey agar5% CO2, 35°C24, 48108–150
Expanded-spectrum EQUC1, 10, and 100BAP, MacConkey agarAerobic, 35°C24, 481–150
BAP, chocolate agar, CNA agar5% CO2, 35°C24, 48
CDC anaerobic BAPAnaerobic, 35°C48
CDC anaerobic BAPbMicroaerophilic gas mixture (5% O2, 10% CO2, 85% N), 35°C48
Streamlined EQUC100BAP, MacConkey agar,c CNA agar5% CO2, 35°C481–150d
  • a Refers to the patient samples on which the corresponding protocol assessed the urinary microbiota. For diagnosis, the standard urine culture protocol was used on patient samples 1 to 107; the modified standard urine culture was used on patient samples 108 to 150. For research, all patient samples were assessed by expanded-spectrum EQUC.

  • b The CDC anaerobic BAP microaerophilic gas mixture condition was used only for samples 10 to 150.

  • c The MacConkey 5% CO2 condition was not part of the expanded-spectrum EQUC protocol.

  • d The streamlined EQUC protocol was performed using a subgroup of agars/conditions from the expanded-spectrum EQUC protocol; therefore, it was used on all patient samples.