Preparation of the SuperPolymyxin medium

CompoundStock solution (mg/ml)Quantity or vol to addaFinal concnb
EMB agar powder15 g3.75%
Distilled water400 ml
Colistin sulfate20 In water in glass tubes70 μl3.5
Daptomycin20 In water200 μl10
Amphotericin B20 In D-(+)-glucose 10%100 μl5
  • a The volume of 400 ml of SuperPolymyxin medium was for, i.e., 20 plates.

  • b Concentrations are in micrograms per milliliter unless noted otherwise.