TABLE 3

Root cause analysis results running the TB-LAMP procedure with only the negative controla

Volume DNA eluent (μl)Temp (°C)Test results (no. false positive/total no.)
Let rxn tubesb sit for 15 min after addition of eluent at tempLet rxn tubesb sit for 15 min at temp and high humidity before addition of eluent
25250/960/48
371/960/48
40NPc2/48
451/963/48
30250/960/48
370/960/48
40NP0/48
450/960/48
  • a High humidity was 85% to 95% relative to temperature.

  • b rxn tubes, TB-LAMP reaction tubes containing reagents that were reconstituted with the addition of the DNA eluent.

  • c NP, combination was not performed.