TABLE 1

Available diagnostic tests for respiratory viruses in seasons 1 and 2

SeasonTest and comments
1 (1 November 2010–31 March 2011)Rapid antigen testing strategy
    Rapid antigen test
        Detection of 3 viruses (influenza A and B viruses, RSV); turnaround time, 15–60 min
    Prodesse ProFlu+ PCR
        Detection of 3 viruses (influenza A and B viruses, RSV); turnaround time, 8–24 h, batched 1–3 times daily (extraction step required over 4 h)
        All negative rapid antigen tests were reflexed to Prodesse PCR due to low sensitivity of rapid antigen test
        During low-prevalence periods (early and late in the respiratory virus season), all positive rapid antigen tests were reflexed to Prodesse PCR
        Rapid antigen testing and Prodesse PCR were the two most commonly used viral diagnostic tests in season 1
Luminex PCR
    Detection of 12 viruses (influenza A virus, influenza A H1 virus, influenza A H3 virus, influenza B virus, parainfluenza 1 virus, parainfluenza 2 virus, parainfluenza 3 virus, RSV A, RSV B, human metapneumovirus, adenovirus, rhinovirus); turnaround time, 48 h (send-out test)
    Used in a minority of patients
Direct fluorescent-antibody testing
    Detection of 3 viruses (influenza A and B viruses, RSV); turnaround time, 2–4 h
    Infrequently performed in our laboratory
Viral culture
    Detection of 8 viruses (influenza A virus, influenza B virus, parainfluenza 1 virus, parainfluenza 2 virus, parainfluenza 3 virus, human metapneumovirus, RSV, adenovirus); turnaround time, 2–10 days
2 (29 February 2012–2 June 2012)FilmArray PCR
    Detection of 15 viruses (influenza A H1 virus, influenza A H1 2009 virus, influenza A H3 virus, influenza B virus, parainfluenza 1 virus, parainfluenza 2 virus, parainfluenza 3 virus, parainfluenza 4 virus, RSV, human metapneumovirus, adenovirus, rhinovirus/enterovirus, coronavirus NL63, coronavirus HKU1); turnaround time, 1–2 h
    Replaced all prior diagnostic methods for respiratory viruses used in season 1
    Individual orders for FilmArray PCR for influenza virus or RSV were used in a minority of patients