TABLE 3

Comparison of pathogens detected by PCR results by quantity levels from 90 MEF samples

Assay resultaNo. (%) of samples
Moraxella catarrhalisHaemophilus influenzaeStreptococcus pneumoniaeAlloiococcusotitidisStaphylococcus aureusPseudomonas aeruginosa
PCR +, any level42 (47)30 (33)27 (30)6 (6.6)5 (5.6)1 (1.1)
    PCR +27 (30)11 (12)10 (11)1 (1.1)4 (4.4)0
    PCR ++15 (16)14 (16)13 (14)4 (4.4)1 (1.1)1 (1.1)
    PCR +++05 (5.5)4 (4.4)1 (1.1)00
PCR −48 (53)60 (66)b63 (70)84 (93)85 (94)89 (99)
Total909090909090
  • a The levels of positivity in PCR were derived from the result of quantitative PCR and are expressed as follows: +++, amplification before cycle 25; ++, amplification in cycle 25 to 31.9; +, any amplification before the end of PCR (45 cycles). All amplification signals were confirmed by direct sequencing of the PCR product.

  • b The data correspond to the sample later detected by the new H_infl_Fv2 primer.