TABLE 1

Primers and probes used for duplex fucP/hpd#3 qPCR

Target genePrimer/probeaSequence (5′ to 3′)Fragment size (bp)Reference
fucPfucP FwdGCCGCTTCTGAGGCTGG6820
fucP RevAACGACATTACCAATCCGATGG
fucP probeb6FAM-TCCATTACTGTTTGAAATAC-BHQ1
hpdhpd#3 FwdGGTTAAATATGCCGATGGTGTTG15116
hpd#3 RevTGCATCTTTACGCACGGTGTA
hpd#3 probecHEX-TTGTGTACACTCCGT/ZEN/TGGTAAAAGAACTTGCAC-3C6
  • a Fwd, forward; Rev, reverse.

  • b Probe was modified with a black hole quencher (BHQ-1) instead of the TaqMan MGB.

  • c Probe was modified with a ZEN internal quencher instead of the BHQ internal quencher.