TABLE 3

Comparison of NTHi culture and fucP/hpd#3 qPCR quantification in clinical specimens

Semiquantitative NTHi culture score (no.)afucP/hpd#3 qPCR geometric mean quantity of DNA (pg/μl) (95% CIb)
+++ (8)6.58 (−4.13 to 30.13)
++ (18)1.45 (0.15 to 8.87)
+ (30)0.18 (0.17 to 2.35)c
0 (23)0.01 (−0.07 to 0.24)d
  • a One culture-positive specimen was excluded from analysis as a semiquantitative culture was not recorded.

  • b 95% CI, 95% confidence interval.

  • c Includes 2 H. influenzae culture-positive specimens that were less than the LOQ for both targets. Specimens in which both targets were not detected were assigned half of the LOQ for statistical analyses.

  • d Two of the culture-negative specimens were qPCR positive.