TABLE 4

F1-LPA normalized signal response of canid Nobuto serum samples compared to immunoblot-based interpretations of F1 reactivity

F1-LPA normalized signal intensityaNo. of samples testedbImmunoblot interpretation (no.)
NegativePositive
1.1×–1.2×404
1.21×–1.3×752
1.31×–1.4×716
1.41×–1.5×716
1.51×–1.6×404
1.61×–2.0×13013
  • a The MFI for each canid Nobuto serum sample obtained using the F1-LPA was normalized to standards S1 and S2 using the following equation: (sample MFI − S1 MFI)/(S2 MFI − S1 MFI). Samples with normalized signal intensities ranging between 1.1× and 2.0× were compared with their respective immunoblotting-based interpretation of F1 reactivity to establish a robust cutoff for a positive sample in F1-LPA.

  • b Following the screening of 3,993 Nobuto samples using the F1-LPA (data not shown), only these 42 (1.05%) samples had normalized signal intensities ranging between 1.1× and 2.0× (Table S4). The average normalized signal intensities for negative and positive samples in the F1-LPA was 0.12× and 2.6×, respectively (Tables S1 and S2).