TABLE 2.

MLST of C. difficile from ELISA-positive stool samples determined both directly using total stool DNA and using isolates cultured from the same stools

Sample no.aELISA ODbMLST PCR result (direct)Direct STcCulture resultiCulture STdPCR ribotype
23+++++++1+++1027
17+0.353+1++1027
2+2.251+1+1027
8+++++++1+++1027
37+1.216+1+1027
43+0.663+1+1027
72+2.5+1+++1027
75+2.5+1++1027
85+0.388+1+1027
88+5.584+1+++1027
89+5.392+1++1027
94+7.098+1+++1027
98+2.5+1+++1027
101+1.208+1+++1027
107+2.5+1+++1027
60+++++++1++1027
53+1.8+2+2014
58+0.261+2++2020
27+++++++2+++2020
11+1.614+2+2020
12+0.968+2+++2020
13+7.718+2+++2020
14+3.648+2++2020
31+5.648+2+2014
41+0.843+2+2020
44+0.29+2+++2020
48+1.003+2++2076
63+2.5+2+++2014
66+2.5+2+++2020
67+2.5+2++2014
84+0.563+2+++2020
1+++++++2++2076
106+1.049+2+++2020
52+++++++3++3072
55+++++++3++3001
57+0.285+3+3072
16+++++++3++3001
64+0.434+3+++3072
65+2.5+4++4137
68+1.322+4++4137
69+2.294+5+++5023
76+2.5+5++5023
78+2.5+5++5023
95+2.5+5+++5023
59+4.083+6+6005
51+++++++6+++6005
26+1.155+6+6005
70+2.5+6++6005
92+1.896+6+++6005
93+0.7+6+++6005
3+0.679+7+7026
32+0.499+7+7026
74+2.5+7+++7026
39+0.27+8+++8002
77+2.427+8+++8002
96+2.282+8+++8002
97+2.379+8+++8002
105+2.136+8+++8002
73+0.499+8+++8002
79+1.163+9+9081
7+0.35+9+9081
80+2.5+10+++10015
99+0.796+10+++10015
15+0.202+10+10015
82+2.5+11++11078
4+2.13+11+++11078
61+0.567+11+11078
20+++++++12+12003
103+0.657+12+++12225
108+2.5+13+++13129
22+++++++14+++14014
5+1.658+14+14014
33+1.658+14++14014
42+0.317+14++14014
46+0.385+14+14014
62+2.5+14+++14014
19+2.257+14+14014
36+1.09+14++14014
28+0.447+19++19110
6+++++++22+22023
49+0.41+37++37017
9+++++++42+++42106
24+0.182+42+42174
40+++++++42++42106
56+3.296+54++54012
104+0.387(+)hNDe+++1027
100+0.67+Mixedg+5023
71+2.322+Mixedg+++11078
45+2.127+1
47+6.186+1
50+0.278+2220
86+0.369+7026
91+3.578+++8002
90+1.155+NDfNDf
102+0.885+NDfNDf
54+0.26
21+7.792
25+0.67
29+3.475
30+0.875
18+0.158
34+1.487
35+0.435
38+0.270
81+0.537
83+0.475
87+0.391
  • a The plus sign indicates an ELISA-positive result.

  • b OD, optical density. +++++, value above the scale.

  • c MLST was performed directly from stool samples.

  • d MLST was performed from cultured isolates.

  • e ND, not done due to very low PCR amplicon yields or no amplicon.

  • f ND, not done because sample was negative on reculturing from frozen isolate stock.

  • g Evidence of more than one genotype present in stool due to mixed peaks in the nucleotide sequence chromatogram.

  • h Poor amplicon yield.

  • i Crude estimate of viable C. difficile load. +, <100 colonies; ++, 100 to 1,000 colonies; +++, >1,000 colonies.