TABLE 1.

PCR primers and products used for sequencing of the Hie capsulation locus

Primer setNucleotide sequence (5′ to 3′)Region amplifiedSize (bp)Source or reference
capfSodCCATGCGCATTTTCCACGCCAGCsodC-bexB1,58119
bexBrevTAGCGATTCAAGGGAGGGTsodC-bexB1,581This study
bexBfrwACGCCCATAACGAGAGACTbexB-bexD2,004This study
bexDrevTCGCAGGTAAGACACCAGAGbexB-bexD2,004This study
bexDfrwAAAGACACCTCGTGGGTCAbexD-regionIII5,003This study
e2GCTTTACTGTATAAGTCTAGbexD-regionIII5,0036
elGGTAACGAATGTAGTGGTAGregionIII-hcsA9,0456
hcsArevACTGACCGCACTTTACGACGregionIII-hcsA9,045This study
hcsAfrwGCACAAAGTGAGCGTCGTAhcsA-hcsB1,703This study
hcsBrevATAGAAGTCTGCCTGGCGAGhcsA-hcsB1,703This study
hcsBfrwGATTGCTTATCGTGGCTCAGThcsB-HI16371,504This study
HI1637AAATTTCCATTATGGGAAACGhcsB-HI16371,50419