TABLE 1.

Biochemical reactions used to identify E. coli species before and after passaging on culture mediaa

AssayReaction of isolate before/after passage on culture medium
ATCC 25922SFPPT 1PPT 2SON 1SON 2SON 3SON 4SON 5
Motility+/++/+−/+−/+−/+−/+−/+−/−−/+
Beta-galactosidase+/++/+−/+−/+−/+−/−−/+−/−−/+
Lysine decarboxylase+/++/+−/+−/++/++/++/+−/++/+
Indole+/++/+−/+−/+−/+−/++/+−/−−/+
Fermentation of sorbitol+/++/++/++/++/++/+−/++/++/+
Tyrosine arylamidase+/++/++/++/+−/++/++/++/+−/+
Alkalinization
    Lactate+/++/++/++/+−/+−/++/+−/−−/+
    Succinate+/++/+−/++/++/+−/++/+−/−−/−
Alkaline phosphatase+/++/+−/++/+−/+−/+−/−−/−−/+
Ellman's esterase assay+/++/++/++/++/++/++/+−/+−/+
  • a SF, synovial fluid; PPT, periprosthetic tissue; SON, sonication.