TABLE 1.

In vitro drug testing of antimalarial activity of T3/SAR97276 and artesunate using different drug sensitivity tests

DrugIncubation period (h)Geometric mean IC50 in nM against P. falciparum 3D7 (95% CI)a as determined by using:
MICHX assaySYBR greenpLDH ELISAHRP2 ELISA
T3/SAR97276485.64.6 (4-5.2)77.3 (25.7-232)>1,00018.5 (14.1-24.3)
7224.5 (3.7-5.6)2.3 (0.6-9.9)19.5 (15.3-24.7)99.3 (50.3-19.6)
72 (w/o drug)b7.28.1 (6.9-9.5)7.4 (5.9-9.2)
96 h1.91.3 (1.2-1.4)3.9 (3.6-4.4)
96 (w/o drug)7.45.3 (4.6-6.3)6.3 (5.7-7)
Artesunate483.71.1 (0.8-1.4)1.9 (1.2-2.8)2.4 (2.2-2.6)1.5 (1.4-1.7)
721.61.2 (0.8-1.6)1.5 (1.3-1.8)2.0 (1.1-3.4)2.5 (1.9-3.3)
72 (w/o drug)1.81.4 (1-2.1)1.5 (1.4-1.7)
961.81.1 (0.9-1.5)1.3 (1.1-1.5)
96 (w/o drug)1.41.4 (1.1-1.8)1.6 (1.5-1.8)
  • a MIC, microscopy; HX, hypoxanthine. Values in boldface do not match the real IC50 (see the text).

  • b w/o drug, without drug. In these cases, after 48 h of contact with the drug, infected erythrocytes were washed for drug clearance and then incubated in fresh drug-free culture medium.