TABLE 2.

Primers used for amplification and sequencing of nine different genomic regions in order to select marker for GCPSR

RegionName of primer, primer sequence (reference), used for:
PCRSequencing
ActinAct-1, 5′-TGGGACGATATGGAIAAIATCTGGCA-3′ (46)Act-1, 5′-TGGGACGATATGGAIAAIATCTGGCA-3′ (46)
Act-4ra, 5′-TCITCGTATTCTTGCTTIGAIATCCACAT-3′ (46)Act-4ra, 5′-TCITCGTATTCTTGCTTIGAIATCCACAT-3′ (46)
β-TubulinB36f modified, 5′-CACCCACTCMCTYGGTGGTG-3′ (41)B36f modified, 5′-CACCCACTCMCTYGGTGGTG-3′ (41)
B12r modified, 5′-CATGAAGAARTGRAGACGVGGGAA-3′ (41)B12r modified, 5′-CATGAAGAARTGRAGACGVGGGAA-3′ (41)
COIM-cox1-fa, 5′-GATATGGCATTTCCTCGAT-3′aM-cox1-fa, 5′-GATATGGCATTTCCTCGAT-3′a
M-cox1-rb, 5′-GGWACTGCAATAATCATTGTAGC-3′aM-cox1-rb, 5′-GGWACTGCAATAATCATTGTAGC-3′a
EF-1αMEF-1, 5′-ATGGGTAAAGARAAGACTCACG-3′ (30)MEF-1, 5′-ATGGGTAAAGARAAGACTCACG-3′(30)
MEF-4, 5′-ATGACACCRACAGCGACGGTTTG-3′ (30)MEF-4, 5′-ATGACACCRACAGCGACGGTTTG-3′ (30)
MEF-11, 5′-AAGAAGATTGGTTTCAACCC-3′ (30)
MEF-21, 5′-GGGTTGAAACCAATCTTCTT-3′ (30)
ITSV9G, 5′-TTACGTCCCTGCCCTTTGTA-3′ (2)V9G, 5′-TTACGTCCCTGCCCTTTGTA-3′ (2)
LR3, 5′-GGTCCGTGTTTCAAGAC-3′ (45)ITS1, 5′-TCCGTAGGTGAACCTGCGG-3′ (47)
ITS4, 5′- TCCTCCGCTTATTGATATGC-3′ (47)
LS266, 5′-GCATTCCCAAACAACTCGACTC-3′ (9)
mtSSUmrSSU1, 5′AGCAGTGAGGAATATTGGTC-3′ (48)mrSSU1, 5′AGCAGTGAGGAATATTGGTC-3′ (48)
mrSSU3r, 5′-ATGTGGCACGTCTATAGCCC-3′ (48)mrSSU3r, 5′-ATGTGGCACGTCTATAGCCC-3′ (48)
nucLSUV9G, 5′-TTACGTCCCTGCCCTTTGTA-3′ (2)NL1, 5′-GCATATCAATAAGCGGAGGAAAAG-3′ (29)
LR3, 5′-GGTCCGTGTTTCAAGAC-3′ (45)LR3, 5′-GGTCCGTGTTTCAAGAC-3′ (45)
RPB1RPB1-Df modified, 5′-TAYAACGCNGATTTCGATGG-3′ (38)RPB1-Df modified, 5′-TAYAACGCNGATTTCGATGG-3′ (38)
RPB1-Fr modified, 5′-CCTTCACGACCACCCATAGC-3′ (38)RPB1-Fr modified, 5′-CCTTCACGACCACCCATAGC-3′ (38)
RPB2RPB2-6f modified, 5′-CCYGCWGAAACKCCMGAAGG-3′bRPB2-6f modified, 5′-CCYGCWGAAACKCCMGAAGG-3′b
bRPB2-7r1, 5′-CCCATRGCYTGYTTMCCCATDGC-3′bbRPB2-7r1, 5′-CCCATRGCYTGYTTMCCCATDGC-3′b