TABLE 3.

Results recorded by culture, RT-PCR, and proposed method for detection of M. tuberculosis in clinical samplesa

Sample codeResult with: No. of gene copies/reactionResult with QD-TB1 probe
CultureRT-PCR
282++13 × 109 +
34++11 × 109 +
323++6 × 109 +
394++4 × 109 +
624++6 × 109 +
146++9 × 109 +
363++13 × 109 +
552++11 × 109 +
287++33 × 103
815++12 × 109 +
531++9 × 109 +
616++7 × 109 +
911++13 × 109 +
77++4 × 109 +
632++8 × 109 +
03++8 × 109 +
286++8 × 109 +
275++16 × 109
34e++7 × 109 +
571++7 × 109 +
1896++2 × 109 +
4813+
687++1 × 109 +
4501++1 × 109 +
1108++600 × 106 +
1077++600 × 106 +
350++11 × 109 +
112++6 × 109 +
132++12 × 109 +
840++16 × 109 +
855++14 × 109 +
220++6 × 109 +
2319++14 × 109
1274++30 × 106
809++320 × 106
2815++1 × 109 +
1345++2 × 109 +
3566++2 × 109 +
633++1 × 109 +
732++1 × 109 +
279++1 × 109 +
674++600 × 106
801++5 × 109 +
2732++800 × 106 +
4464++3 × 109 +
4354++700 × 106 +
4500++3 × 109 +
1818++3 × 109 +
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5
6
7
8
9
10
11
12
  • a Real-time PCR and proposed assay for detection of M. tuberculosis from samples of bronchoalveolar lavage collected from patients with tuberculosis and from healthy individuals.