TABLE 4.

Master mix components for serovar-specific monoplex PCR assays

SerovarConcn (μM) of:PCR bufferaMgCl2 concn (mM)bUNG amt (U)
Forward primerReverse primerProbe 1Probe 2
10.30.50.220.5
20.50.513.00.5
30.250.2513.00.5
40.50.50.20.220.5
50.40.50.220.5
60.40.413.00.5
70.250.2513.00.5
80.250.2513.00.5
90.250.2513.00.5
100.30.50.1520.5
110.20.50.220.5
120.50.50.20.220.5
130.40.413.00.5
140.50.513.00.5
  • a 1, 2 μl LightCycler FastStart DNA Master SYBR green I; 2, 4 μl LightCycler FastStart DNA MasterPLUS HybProbe.

  • b Final MgCl2 concentration.