TABLE 4.

Results recorded by ZN, culture, RT-PCR, and proposed method for detection of M. avium subsp. paratuberculosis in clinical samplesa

Sample codeResult with: DNA concn (ng/reaction)Result with QD-MAP3 probe
ZN stainCultureRT-PCR
43+NA+0.113
44+NA+0.128+
54+NA+36.56+
68+NA+2.317+
69+NA+0.27
141+NA+0.08
142+NA+0.059
332+NA16.5+
326+NA+0.18
524+NA+0.13
525+NA+90.07+
526+NA+19.2+
728+NA+0.047
735+NA
828+NA+0.0214
681+NA+54.54+
66+NA
729+NA+210.05+
731+NA+1054.83+
756+NA+1.432+
757+NA+26.05+
71+NA+2.65+
104+NA+57.19+
660+NA+0.025
661+NA+4.657+
829+NA
1NA
2NA
3NA
4NA
5NA
6NA
7NA
1NAb ++1741.9+
2NA++154.92+
3NA++1552.31+
4NA++240.68+
5NA++0.32
6NA++14.34+
7NA++0.458
8NA++1.034+
9NA++584.89+
10NA++38.08+
11NA++0.04
12NA++4468.98+
13NA++1258.12+
14NA++4718.01+
15NA
16NA
17NA
18NA
19NA
20NA
  • a Real-time PCR and the proposed assay for the detection of MAP in fecal and FFPE samples collected from goats with paratuberculosis and healthy animals.

  • b NA, not applicable.