TABLE 2.

Viable-aerosol generation during manual sample preparation

Sputum preparation methodCFU/m3 airb
Andersen impactorBioSampler
SR added to sputum, then cartridge loaded without incubationa 6 ± 5.567 ± 115.4c
SR added to sputum, then cartridge loaded after 15-min incubationa 00
Sputum smeared directly on microscope slides; no SR16 ± 2.3325 ± 112.5
  • a After SR addition, samples were pipetted in and out of three Xpert MTB/RIF cartridges (equivalent to loading >30 cartridges).

  • b Mean and standard deviation of three experiments.

  • c Two of the three replicates did not show any growth in the 7H9 medium.