Test location and Quansys ELISA result (type of antigens identified)^{b} | No. of samples with uPCR result (type of DNA identified)^{b} | |||
---|---|---|---|---|

None | P. vivax | Plasmodium spp. | P. falciparum | |

Laboratory | ||||

None | 3^{c} | 1^{c} | 0^{c} | 2^{f} |

P. vivax (PvLDH) | 0^{c} | 1^{c} | 0^{c} | 0^{f} |

P. falciparum (PfHRP2) | 2^{d} | 0^{e} | 4^{e} | NA |

P. falciparum and P. vivax (PfHRP2 plus PvLDH) | 0^{d} | 1^{e} | 2^{e} | NA |

Plasmodium spp. (PanLDH only) | 0^{c} | 0^{c} | 0^{c} | 2^{f} |

Field | ||||

None | 1^{c} | 1^{c} | 0^{c} | 0^{f} |

P. vivax (PvLDH) | 0^{c} | 2^{c} | 0^{c} | 1^{f} |

P. falciparum (PfHRP2) | 0^{d} | 1^{e} | 3^{e} | NA |

P. falciparum and P. vivax (PfHRP2 plus PvLDH) | 0^{d} | 0^{e} | 0^{e} | NA |

Plasmodium spp. (PanLDH only) | 0^{c} | 0^{c} | 0^{c} | 1^{f} |

↵a A color-coded version of Table 3 is available in the supplemental material (Table S1).

↵b See footnotes

*c*to*f*below. Values with footnotes*c*and*d*are false-positive results by reference C (*n*= 7 for laboratory and*n*= 4 for field). Values with footnotes*c*and*f*are false-positive results by reference A (*n*= 9 for laboratory and*n*= 6 for field). Values with footnotes*c*,*d*, and*e*are false-positive results by reference B (*n*= 14 for laboratory and*n*= 8 for field). NA, not applicable (corresponds to PfHRP2-positive and PfDNA-positive samples [true positive according to all references]).↵c P. falciparum-negative samples by all three reference methods. These are most likely false-positive uRDT results.

↵d PfHRP2-positive, PfDNA-negative, and Plasmodium DNA-negative samples (positive by reference A only). These two samples could correspond to recently cleared infections with persisting HRP2 antigenemia.

↵e PfHRP2-positive, PfDNA-negative, and Plasmodium DNA-positive samples (P. falciparum positive by reference A and C).

↵f PfHRP2 negative and PfDNA positive (P. falciparum positive by reference B and C).