Table 2.

Primers used to characterize Tn1546 from vancomycin-resistant enterococcia

PrimerSequence (5′→3′)Position
VanA gene cluster
 Van A1AAA TGT GCG AAA AAC CTT GC7127–7146
 Van A2AAC AAC TAA CGC GGC ACT7662–7645
 VanS1ATT GTT CAG CAT GGA GGG C5696–5714
 VanH2GAG CAT GGA ATG CAT CTG CC6081–6063
 VanR1AAA TAA GGG ACA AGC ACA CC4125–4194
 VanR2CCC ATA TCT CAT GAA ATA GC4534–4515
 VanX1ACT TGG GAT AAT TTC ACC GG8082–8101
 VanX2TGC GAT TTT GCG CTT CAT TG8505–8486
 VanX3CTC ATC ATG CGG CAA ATG G8458–8476
 VanY1TGG GTA TTT TCA GAA GTC CC9212–9193
 VanY2-1GTT TCC CGG ATC AAC ACA TAC TA9927–9949
 VanZ2-2CCC AGT AGC AGT AAA TGG AGT CA10262–10240
 VanZ1CTG GGA ATT TCA GAG AGA TG10258–10277
 VanZ2AAT GGG TAC GGT AAA CGA GC10581–10562
 ORF12CCA TTC CTC GTA TGT ATT CG2318–2337
 VanR2CCC ATA TCT CAT GAA ATA GC4534–4515
 ORF14-2GCC CTT AGG TTG GGA ACA TA174–155
 VanSma-2ATA AAA TGA CTA ACG CCA CC9446–9427
IS1216V– IS3-like
 IS1216V-1AAA GCA ATT TCA GCA GGA TG256–275
 IS1216V-2GTA CGA TGT TCT GTC CCT TG711–692
 IS3-like-1ACT GGG TAT CGC CAA ATC CA1251–1270
 IS3-like-2TTT GTC CCA TTG GTC AAC CG1619–1600
 IS3-like-2-1CCA CAC TGA TTC ATA GCG AC1748–1767
IS1251
 IS1251-3GCA TCC ACT GTA AAC ACC AG273–292
 IS1251-4CGC TGT GTT TGA CCA TCC AT699–680
IS1476, IS1476-2-1CTT TCG GGC ACG GAT CTA TT1366–1385
  • a All primers were used for PCR amplification of internal areas of Tn1546. Positions refer to sequences published in GenBank; access numbers are M97297 for Tn1546, L40841 for IS1216V, L34675 for IS1251, and U63997 for IS1476. Correct positions of the IS sequences were determined by using one primer inside the IS sequence and one in the flanking region of Tn1546. For the position of IS1251, primers VanH2 and IS1251-4 were used; for linking of IS1216V to the IS3-like element, primers IS1216V-1 and IS3-like-2 were used; and IS3-like-2-1 and ORF14-2 were used to position this complex in the left end of Tn1546. Primers IS1476-2-1 and VanSma-2 were used to determine whether IS1476 was present.