Table 1.

PCR primers

PrimerSequence (5′–3′)SpecificityaAmplicon size (bp)
Assay 1
 stx1FATAAATCGCCATTCGTTGACTACnt 454–633 of A subunit coding region ofstx 1 180
 stx1RAGAACGCCCACTGAGATCATC
 stx2FGGCACTGTCTGAAACTGCTCCnt 603–857 of A subunit coding region of stx 2 (includingstx 2 variants)255
 stx2RTCGCCAGTTATCTGACATTCTG
 eaeAFGACCCGGCACAAGCATAAGCnt 27–410 ofeaeA (this region is conserved between EPEC and STEC)384
 eaeARCCACCTGCAGCAACAAGAGG
 hlyAFGCATCATCAAGCGTACGTTCCnt 70–603 of EHEChlyA 534
 hlyARAATGAGCCAAGCTGGTTAAGCT
Assay 2
 O157FCGGACATCCATGTGATATGGnt 393–651 of rfbE O157:H7 259
 O157RTTGCCTATGTACAGCTAATCC
 O111FTAGAGAAATTATCAAGTTAGTTCCnt 24–429 of ORF 3.4 of E. coli O111 rfb region406
 O111RATAGTTATGAACATCTTGTTTAGC
  • a nt, nucleotide; ORF, open reading frame.