Table 3.

Genetic characterization of Tn1546-like elements from vancomycin-resistant enterococci by PCR and DNA hybridization

Isolate typeaIsolate no.SourcePCR resultDNA hybridization signald(kb)
MvanXbIS1216V–IS3-likecIS1251cVanR (H3)VanX
BHI-KIBHI-BIIBHI-EV
11PonyG0073.52.5
12DuckG0073.52.5
13ChickenG0073.52.5
14TurkeyG0073.52.5
113HumanG0073.52.5
127HumanG0073.52.5
130BroilerG0073.52.52.1
133BroilerG0073.52.5
134BroilerG0073.52.5
138BroilerG0073.52.5
28PigT1073.52.5
29PigT1073.52.5
210PigT1073.52.52.1
214HumanT1073.52.5
228HumanT1073.52.52.1
231PigT1073.52.5
232PigT1073.52.5
235PigT1073.52.5
236PigT1073.52.5
239PigT1073.52.5
240HumanT1073.52.5
319HumanG0083.010.510.5
320HumanG0083.010.510.5
321HumanG0083.010.510.5
322HumanG0083.010.510.5
323HumanG0083.010.510.5
326HumanG0083.010.510.5
45HumanG1073.52.5
56HumanG107, 93.5, 4.42.5, 3.52.1, 2.9
67HumanG1073.52.5
711HumanG0083.010.52.1
815HumanT014, 53.52.5
916HumanT1143.52.5
917HumanT1143.52.52.1
1024HumanG008, 113.0, 7.05.5, 10.56.8, 10.5
1125HumanG00154.43.52.9
1229BroilerG0084.43.52.9
1337HumanT0073.52.5
  • a Isolates are divided into 13 types according to the results obtained.

  • b Sequence result of PCR-amplified region of the vanX gene. T, sequence homologous to published sequence of Tn1546; G, sequence with a single-nucleotide variation (T to G) at position 8234.

  • c Presence of the IS1216V–IS3-like element and of IS1251 at the published positions in the left end of Tn1546 and in the vanSH intergenic region, respectively. Symbols: 1, correct position; 0, absence of a positive PCR amplification.

  • d Where two values are given, two bands were detected. Abbreviations: H3, HindIII; BHI,BamHI; KI, KpnI; BII, BglII; EV,EcoRV.