Table 1.

Sequences of oligonucleotide primers used for the amplification and early detection of human CMV genome

Gene regionPrimer pairSequenceProduct length (bp)Refer- ence
HindIII-X fragmentP15′-GGATCCGCATGGCATTCACGTATGT-3′406 3
P25′-GAATTCAGTGGATAACCTGCGGCGA-3′
EcoRI fragment DP15′-GATCCGACCCATTGTCTAAG-3′152 17
P25′-GGCAGCTATCGTGACTGGGA-3′
IEA1 geneP15′-CAAGCGGCCTCTGATAACCAAGC-3′438 11
P25′-CTCTTCCTCTGGGGCAACTTCCTC-3′
MIE geneP15′-GGGTGCTGTCCTGCTATGTCTTA-3′370 4
P25′-CATCACTCTGCTCACTTTCTTCC-3′