Table 1.

Sequences of primers used for detection of different genes by the PCR assay

GenePrimer sequenceAmpli- con size (bp)Refer- ence
ctxA CTC AGA CGG GAT TTG TTA GGC ACG 301 16
TCT ATC TCT GTA GCC CCT ATT ACG
zot CAC TGT TGG TGA GCG TTA TCG 243 6
CAA GCG CTG TGG GTA GAA GTG AAA
ace GCT TAT GAT GGA CAC CCT TTA 284 6
TTT GCC CTG CGA GCG TTA AAC
tcpA(El Tor) GAA GAA GTT TGT AAA AGA AGA ACA C 471 16
GAA AGG ACC TTC TTT CAC GTT G
tcpA(classical) CAC GAT AAG AAA ACC GGT CAA GAG 617 16
ACC AAA TGC AAC GCC GAA TGG AG
LTc GCG ACA AAT TAT ACC GTG CT 708 33
CCG AAT TCT GTT ATA TAT GT
STc ATT TTT C/ATT TCT GTA TTA/G TC 105 30
GC/TA CAG/A GCA GGA TTA CAA AA
VTc GAA CGA AAT AAT TTA TAT GTG 521 32
TGA TGA TGG CAA TTG AGT AT