Table 2.

Between-run reproducibility of bDNA and RT-PCR for quantitation of HIV-1 RNA copies per milliliter at two concentrationsa

SamplebMethodMeanSDCV (%)
1bDNA297,06672,20524.3
RT-PCR780,826267,93734.3
2bDNA24,01110,57144.0
RT-PCR110,84347,32242.7
  • a Each sample was tested in triplicate in six separate runs for a total of 18 replicates.

  • b Samples 1 and 2 were 1.5 × 106- and 1.5 × 107-fold dilutions, respectively, of an HIV-1 SF2 stock suspension quantitated at 2.48 × 109 virus particles/ml.