TABLE 2.

Adapters and primers used

Oligonucleotide nameSequence (5′→3′)Selective baseEnzyme end
Adaptersa
    924ACTGCGTACCN/AEcoRI
    925AATTGGTACGCAGTCAGTGAGGTTCATTACCATCCN/AEcoRI
    930TACTCAGGACTCN/AMseI
    931CCTGATTGCTACAACTGAACGATGAGTCCTGAGN/AMseI
Preamplification primers
    926GGATGGTAATGAACCTCACTNoneEcoRI
    932CCTGATTGCTACAACTGAACNoneMseI
Selective primers
    927GACTGCGTACCAATTCAAEcoRI
    1269GACTGCGTACCAATTCTTEcoRI
    1270GACTGCGTACCAATTCGGEcoRI
    1271GACTGCGTACCAATTCCCEcoRI
    933GATGAGTCCTGAGTAACCMseI
    1272GATGAGTCCTGAGTAAAAMseI
    1273GATGAGTCCTGAGTAATTMseI
    1274GATGAGTCCTGAGTAAGGMseI
  • a See reference 28 for details on adapter preparations.