TABLE 4.

Comparison of real-time and nested PCR assays with cell culturing for detection of C. pneumoniae in 179 oropharyngeal specimens

No. (%) of oropharyngeal specimensResulta of:
CulturingbReal-time TaqMan16S rRNA nested PCRompA nested touchdown PCR
VD2VD4
2 (1.1)+++
2 (1.1)+
1 (0.6)++
2 (1.1)Overgrowthc+
1 (0.6)+
1 (0.6)Overgrowth+
2 (1.1)+
6 (3.3)Overgrowth
162 (90.5)
  • a +, positive; −, negative.

  • b One to three chlamydial inclusions were visualized in three wells. Only one of the positive cultures could be propagated after three blind passages. The other three positive cultures could not be propagated, and results were confirmed by PCR analysis of the infected monolayers.

  • c Overgrowth by respiratory flora.